One-two punch treatment delivers blood cancer knockout

The WEHI research team paired venetoclax, a current standard-of-care anti-cancer drug for AML, with a STING agonist, an emerging class of immunotherapy drugs. Venetoclax was based on a landmark research discovery at WEHI.

Study co-first author Dr Sarah Diepstraten said the team examined a range of different blood cancers, including cancer samples from patients with AML and treated them in the lab with the drug combination, leading to striking results.

“It’s really impressive – combining venetoclax with this emerging immunotherapy treatment can actually eradicate AML,” said Dr Diepstraten.

“This is the one-two punch combo that could be the knockout blow for AML. You could almost paraphrase the famous boxer Muhammed Ali and say this treatment floats like a butterfly, and STINGs like a bee.”

Critically, the combination treatment showed high promise in AML samples that were driven by a mutated p53 protein, a type of AML that is generally more aggressive and harder to treat.

The p53 protein plays a critical role in our bodies. When it is working effectively, it prevents the formation of cancerous cells by enforcing the death, or arresting the growth, of cells that have become damaged or abnormal.

But when the p53 protein is mutated and becomes defective in groups of cells, it can significantly boost a person’s risk of developing cancer.

Mutations of p53 are thought to be the biggest driver of cancer development and are found in half of all human cancers around the globe.

As cancers associated with p53 mutations tend to be more aggressive and resistant to treatment, there is a critical need for better therapies.

“For AML patients that do not have as much therapy-induced death of their leukaemia cells due to this mutated protein, combining venetoclax with a STING agonist causes more killing of AML cells than treating with venetoclax alone,” said Dr Diepstraten.

“The treatment was highly effective at killing cancer cells in samples with and without the p53 mutation, which is exciting given the lack of effective treatments for aggressive cancers driven by mutations in p53.

“We want to see more and more patients getting into long-term remission from blood cancers like AML, and we think adding STING agonists to the treatment regimen could be the key.”

The study marks the first time that a STING agonist has been used to directly target mechanisms within the cancer cells themselves, stimulating the natural processes that cause these cells to die.

To date, the STING agonist immunotherapy drugs have been chiefly used to attack solid tumours by activating the body’s immune response.

The new research builds our understanding of how STING agonists work and indicates for the first time that they could be successful in ways that had not been previously anticipated: against blood cancers, and by directly targeting the cancer cells intrinsically.

Associate Professor Gemma Kelly, co-senior author on the study, said that both venetoclax and the STING agonists played complementary roles in killing cancer at the cellular level.

“Within a cancer cell, venetoclax blocks the machinery of the cell that is keeping it alive. In certain blood cancers where this response is sub-optimal, STING agonists can supercharge this effect to deliver cancer a deathly blow,” Assoc Prof Kelly, a laboratory head in WEHI’s Blood Cells and Blood Cancer division, said.

“It is these two drugs, working in tandem, that led to the highly effective killing of AML cancer cells in our lab-based tests, in results that were truly striking.”

WEHI lab head Professor Andrew Wei, co-senior author on the study, said that while further research was needed, the findings were highly promising.

“While early clinical trials in solid cancers have suggested STING agonists are well tolerated in the body, these results offer exciting new hope for patients with the most resistant forms of leukaemia,” said Prof Wei.

“Given STING agonists are currently in clinical trials, we hope to conduct human studies using STING agonists in combination with venetoclax in the near future.

“The research findings are important, as they will inspire a completely new clinical approach for patients affected by the most resistant and deadly forms of acute leukaemia.”

WEHI researchers and clinicians are now translating these highly promising findings into a new clinical trial in AML patients in collaboration with Melbourne-based Aculeus Therapeutics, a local biotechnology company with a proprietary STING agonist.

Dr Mark Devlin, CEO of Aculeus Therapeutics, which has spent the last several years developing a potent STING agonist, couldn’t be more excited about the potential for the recent WEHI discovery.

“Drug discovery and development is the ultimate scientific team sport,” he said.

“Aculeus has developed a promising new drug but the collaboration with the WEHI teams, that have a deep understanding of the disease biology and the clinical landscape, will help shape how that drug will be used most effectively.”

Aculeus’ STING agonist, ACU-0943, is expected to enter clinical development for the treatment of AML later this year.

This research was supported by the VCA, NHMRC, Medical Research Future Fund, CASS Foundation, the estate of Anthony (Toni) Redstone OAM, the Craig Perkins Cancer Research Foundation, the Dyson Bequest, the Harry Secomb Foundation, Robert and Janette Boffey, Jill and Stuart Bales, Metcalf Family Fellowship, RCPA Foundation and operational infrastructure grants through the Victorian State Government Operational Infrastructure Support (OIS) and Australian Government NHMRC Independent Research Institute Infrastructure Support (IRIIS) Schemes.

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